Fluidigm Access Array Service Details
Project and Assay Design Workflow We will first have a meeting to discuss your research goals and the suitability of the Fluidigm Access Array technology in achieving those goals. If this platform is applicable, a CSAS will be submitted, an estimate for total project cost will be generated, and after CSAS approval, we will order the appropriate reagents from Fluidigm and begin processing your samples.
Sample Processing Workflow When a request is approved, we will order primers that will be validated and optimized using control DNA prior to amplification of customer samples. Once samples are received, LMT staff will determine the concentration via either NanoDrop or PicoGreen. Specimens will then be normalized and processed through our QC workflow again. The samples, in multiples of 48 (samples and regions), will have amplicons generated on Fluidigm’s Access Array. These amplicons can be returned to the customer or proceed directly to CORE service sequencing. Depending on the next generation sequencing platform chosen for your run, amplicon libraries will be prepared at the LMT or the Sequencing Facility, followed by either a Roche 454, Ion Torrent, or Illumina HiSeq, MiSeq, or GAII run. Sequence run data are sent to the Advanced Biomedical Computing Center (ABCC) for further QC and analysis. The customer is notified from ABCC when there is data to review.