Microarray technology can be used to measure expression of tens to thousands of genes simultaneously, determine copy number variation, and global SNP genotyping. By using commercially-available microarrays, which contain nucleotide sequences complimentary to regions of interest, expression level is determined by differences in fluorescence intensity at each location of the microarray. These microarray platforms are performed using single-color or dual-color arrays. In single-color arrays, all samples and controls are labeled with the same fluorescent dye and one sample is hybridized to one array. In dual-color arrays, samples are commonly labeled with one fluorescent dye and controls with another dye, and both sample and control are co-hybridized to one array.
Microarray services can be applied to your research to:
- Measure expression and regulation of protein-coding transcripts
- Measure expression of non-coding RNA (miRNA)
- Determine copy number variation of genes
The Affymetrix technology is ideal for measuring gene expression for thousands of genes in a high-throughput environment, and numerous microarray chips for human and mouse are available for your research. However, if your research suggests a more targeted approach to gene expression, the NanoString technology is ideal for measuring tens to hundreds of genes, including custom design of assays for your transcripts of interest.
For more information on this service and its applications to your research, visit our Microarray Protocols and Resources page.