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Roche 454 Project and Sample Submission Requirements

Roche 454 Project and Sample Submission Requirements

Project Requirements

All requests for scientific and analytical work must be submitted through CSAS.  Please contact Kristen Pike for more information regarding submitting requests, costs, and additional sources of funding for your project.

Sample Requirements

The table below summarizes the minimum requirements for assay processing.  We recommend that samples be quantified via Nanodrop or PicoGreen prior to normalization and shipment to the Laboratory of Molecular Technology.  Since efficient and reliable processing of your samples is important to us, we would appreciate if you could send us more volume and more concentration of sample as your specimen source allows.  This will enable proper sample QC and ensure that enough material is present if evaporation occurs during shipment, or it becomes necessary to repeat a reaction.

Description of Service Sample Type Min.Vol. per reaction (uL) Min. Conc. (ng/uL) Total DNA per PCR reaction (ug) Format Comments
Roche FLX Titanium 454 next generation sequencing PCR products >20 ul >5 ng/ul > 100 ng 1.5 or 2.0 mL sterile polypropylene screw cap tubes or 96 well plates Recommended tubes are from Sarstedt, catalog numbers 72.692.005 or 72.694.006 or Pantek 96 well plates, part number 99990040bc
Roche FLX Titanium 454 next generation sequencing genomic DNA,  BACs and cDNA >50-100 ul >20-50 ng by fluorometry or 80-160 ng by Nanodrop >1 ug – 2 ug by fluorometry or 4-8 ug by Nanodrop 1.5 or 2.0 mL sterile polypropylene screw cap tubes or 96 well plates Recommended tubes are from Sarstedt, catalog numbers 72.692.005 or 72.694.006 or Pantek 96 well plates, part number 99990040bc

Shipping Information

Subsequent to CSAS approval, please send samples along with a copy of your sample manifest to the Laboratory of Molecular Technology through the NCI-Fredrick courier.  To schedule pickup, call transportation at (301)-846-1175 and provide them with the pickup location and the following drop-off information:

  • Contact Name:  Roche 454 Group
  • Building and Room Number:  ATRF, 8560 Progress Drive, Wing C, 3rd Floor
  • Phone Number:  (301)-846-5676
  • Type of Shipment: DNA 

 

Page last updated May 21, 2013 @ 9:17 am