SureSelect Project and Sample Submission Requirements
Project Requirements
All requests for scientific and analytical work must be submitted through CSAS. Please contact Kristen Pike for more information regarding submitting requests, costs, and additional sources of funding for your project.
Sample Requirements
The table below summarizes the DNA requirements for efficient and reliable processing of your samples. We recommend that samples be quantified via either Nanodrop or PicoGreen prior to normalization and shipment to the Laboratory of Molecular Technology.
|
Description of Service |
Sample Type |
Min.Vol. per reaction (uL) |
Min. Conc. (ng/uL) |
Total DNA per PCR reaction (ug) |
Format |
Comments |
|
SureSelect capture and library preparation |
DNA |
60 ul |
60 ng/ul |
3.6 ug |
1.5 or 2.0 mL sterile polypropylene screw cap tubes or 96 well plates |
Recommended tubes are from Sarstedt, catalog numbers 72.692.005 or 72.694.006 or Pantek 96 well plates, part number 99990040bc |
Shipping Information
Subsequent to CSAS approval and placing a request for service in the LMT Molecular Diagnostics Wizard, please send samples along with a copy of your Wizard generated sample manifest to the Laboratory of Molecular Technology through the NCI-Fredrick courier. To schedule pickup, call transportation at (301)-846-1175 and provide them with the pickup location and the following drop-off information:
- Contact Name: Kristen Pike or Mutation Detection Group
- Building and Room Number: 915 Tollhouse Ave. Suite 211
- Phone Number: (301)-846-5676
- Type of Shipment: DNA
