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SureSelect Project and Sample Submission Requirements

Project Requirements

All requests for scientific and analytical work must be submitted through CSAS.  Please contact Kristen Pike for more information regarding submitting requests, costs, and additional sources of funding for your project.

Sample Requirements

The table below summarizes the DNA requirements for efficient and reliable processing of your samples.  We recommend that samples be quantified via either Nanodrop or PicoGreen prior to normalization and shipment to the Laboratory of Molecular Technology.

Description of Service

Sample Type

Min.Vol. per reaction (uL)

Min. Conc. (ng/uL)

Total DNA per PCR reaction (ug)

Format

Comments

SureSelect capture and library preparation

DNA

60 ul

60 ng/ul

3.6 ug

1.5 or 2.0 mL sterile polypropylene screw cap tubes or 96 well plates

Recommended tubes are from Sarstedt, catalog numbers 72.692.005 or 72.694.006 or Pantek 96 well plates, part number 99990040bc

Shipping Information

Subsequent to CSAS approval and placing a request for service in the LMT Molecular Diagnostics Wizard, please send samples along with a copy of your Wizard generated sample manifest to the Laboratory of Molecular Technology through the NCI-Fredrick courier. To schedule pickup, call transportation at (301)-846-1175 and provide them with the pickup location and the following drop-off information:

  • Contact Name:  Kristen Pike or Mutation Detection Group
  • Building and Room Number:  ATRF, 8560 Progress Drive, Wing C, 3rd Floor
  • Phone Number:  (301)-846-5676
  • Type of Shipment: DNA

Page last updated May 21, 2013 @ 9:18 am