EML Services
The Electron Microscopy Laboratory (EML) offers a variety of core services. To order services, please submit an electronic request via CSAS; or to discuss the project scope and design, please contact Dr. Ulrich Baxa. For additional information on how to use the on-line accession system please click here. EML services include preparation and shipment of sample fixative, sample processing, embedding, sectioning, staining and imaging. The EML has a number of resources and protocols that may also be helpful.
|
Research Need |
Technique |
Instrument |
|
|
+++ = Best
++ = Good + = Poor - = Not Available |
Please see EML services and technology development for more information on techniques. |
SEM |
TEM |
Surface Analysis of cells and soft matter nanoparticles
|
Processing and metal coating for SEM
Energy dispersive x-ray spectroscopy |
+++ +++ |
- ++ |
Surface Imaging of large biological objects and materials
|
Variable pressure SEM with cooling stage |
+++ |
- |
Virus Particle Analysis
|
Plastic embedding/ thin section TEM
Negative staining Cryo-EM |
- - - |
+++ +++ +++ |
Elemental Analysis
|
Energy dispersive x-ray spectroscopy
Selected Area Electron Diffraction |
+++ - |
+++ +++ |
Ultrastructure
(Subcellular/Organelles) |
Plastic embedding/ thin section TEM |
- |
+++ |
Protein complexes and isolated organelles
|
Negative staining
Plastic embedding/ thin section TEM Cryo-electron microscopy |
- - - |
+++ +++ +++ |
Nanoparticle Analysis
|
Drop cast on appropriate film
Energy dispersive x-ray spectroscopy |
++ +++ |
+++ +++ |
Soft Matter Nanoparticle Analysis
|
Cryo-EM
Negative staining |
- - |
+++ +++ |
Nanoparticles in Cells or Tissues
|
Plastic embedding/ thin section TEM
Energy dispersive x-ray spectroscopy |
- + |
+++ +++ |
3-Dimensional Analysis of cellular ultrastructure or nanoparticles
|
Electron tomography from thin plastic sections
Electron tomography from cryo-EM specimens |
- - |
+++ +++ |
|
Immuno Electron Microscopy
|
Pre-embedding immunolabeling
Post-embedding immunolabeling Immuno-labeling followed by negative staining Immuno-labeling followed by SEM |
- - - +++ |
+++ +++ +++ - |
Custom Project
|
Please contact us about customized protocols or how to process and image unique samples. | ||
