Image Gallery
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Fixed MEF cells staining Actin (red) and Tubulin (green).
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Fixed MEF cells staining Golgi (red) and nucleus (cyan). Samples prepared and imaged by Dr. Luana Scheffer, laboratory of Dr. Jairaj Achyria, CCR, NCI-Frederick.
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Live MEF cells staining Golgi apparatus (green) and Mitochondria (red).
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3D rendering of the node of a mouse embryo. The red staining is F-actin, the green is antibody labeling of acetylated tubulin to visualize cilia, and the cyan is cell nuclei. The sample preparation and labeling were performed in the laboratory of Dr. Terry P. Yamaguchi, Cancer and Developmental Biology Laboratory, Center for Cancer Research, National Cancer Institute. Image acquisition and 3D rendering were performed in the laboratory of Dr. Stephen Lockett, Advanced Technology Program, National Cancer Institute/SAIC-Frederick.
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FluoCells® prepared slide #1 (F36924) contains bovine pulmonary artery endothelial (BPAE) cells stained with a combination of fluorescent dyes. Mitochondria were labeled with red-fluorescent MitoTracker® Red CMXRos, F-actin was stained using green-fluorescent Alexa Fluor 488 phalloidin, and blue-fluorescent DAPI was used to label the nuclei.
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Drosophila Kidney RC cells. Red channel is antibody-labeled Scrib in RC cells, one of the tumor suppressors in Drosophila) antibody-labeled in Drosophila Kidney RC cells; Green is Stat-GFP, which labels the kidney stem cells (RNSC) and transit cells (RB); Blue is DAPI. Sample prepared and imaged by Dr. Xiankun Zeng, laboratory of Dr. Steven Hou, CCR, NCI–Frederick and rendered with IMARIS.
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Five-color FISH imaging in mouse embryonic stem cells to study strand segregation of Chromosome 11 through mitosis. DNA (blue), Centromere (orange), Chromosome 11 (White), G repeat Telomeres (green), C repeat Telomere (red). Metaphase spread was prepared so that one sister chromatid is single stranded. Sample prepared and imaged by Dr. Stephan Sauer, laboratory of Dr. Amar Klar, CCR, NCI-Frederick
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Drosophila prostate. A) Escargot-GFP labeling a subset of cells. B)DAPI nuclear staining. C) Red: phalloidin labeling F actin. D) Overlay. Sample prepared and imaged by Dr. Shree Ram Singh, laboratory of Dr. Steven Hou of CCR, NCI-Frederick (unpublished).
