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OMAL Microscopes Recommended Laser Settings

Recommended Laser Settings (510 Meta) Laser
405 10-40% not > 50%
458 40-50% to start – can go to 70%. (It is a weak laser.)
477 weak laser.
488 10-15% (unless you want to photobleach.); 5-7% is often enough.
514 15-20-25% (Maybe 30% but it is a strong laser.)
561 30-40% is sufficient. It is much stronger than the 543 nm laser.
594 30-40% recommended. It is a strong laser.
633 30-40%. Occasionally 50% may be necessary.
Recommended Laser Settings for NLO510 (Building 538)
Laser
458 30%
488 1%
514 1%
543 97%. Weak laser.
633 5-10%
755 No higher than 25% or you can burn your sample.

Page last updated November 9, 2010 @ 6:57 pm