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Microscopes at NCI Frederick

Microscope Capabilities

Zeiss LSM 710 Confocal Microscope in B560

  • Laser lines: 405, 458, 488, 514, 561, 594, 633, 690-1064
  • Environmental chamber for long-term live cell experiments

Zeiss LSM NLO510 Confocal Microscope in B538

  • Visible laser lines: 458, 477, 488, 514, 543 and 633 nm
  • InfraRed laser: 700 to 1000 nm for live cell and deep tissue imaging
  • Detectors: Three in the epi-position and two non-descanned detectors for improved two-photon sensitivity and second harmonic generation imaging
  • Image acquisition rate: 5 full images per second, faster for sub-regions
  • META detector for spectral image acquisition, i.e. when fluorescence labels have overlapping emission spectra
  • 8 well environmental chamber for live cells: Control of temperature and CO2 and media for live cells
  • Microinjection

Zeiss LSM UV510 Confocal Microscope in B560

  • Visible laser lines: 405, 458, 477, 488, 514, 561, 594 and 633 nm
  • META detector for spectral image acquisition
  • Low light camera for conventional imaging, plus deconvolution software

Zeiss LSM UV510 Confocal Microscope in B560

  • Visible laser lines: 405, 458, 477, 488, 514, 561, 594 and 633 nm
  • Environmental chamber for long-term live cell experiments

All Zeiss LSM 510s

  • Computer-controlled stage for large area imaging
  • Reflection, DIC and phase imaging
  • Data Archiving: Images immediately available via www and automatic back up

Olympus FV1000 Confocal Microscope in B538

  • Visible laser lines at: 405, 458, 488, 514, 561 and 633 nm
  • Computer controlled stage
  • Photon Counting Mode
  • Environmental chamber for long-term live cell experiments

TIRF and Super-resolution imaging in B538

  • Live cell TIRF imaging
  • Super-resolution PALM imaging: fixed cells labeled with photoactivatible fluorescent proteins
  • Super-resolution SIM imaging in conjunction with NIH imaging lab: fixed cells with any normal fluorescent dyes 
  • Please call for service

Microscope Applications

  • Five color image acquisition
  • Time-lapse imaging
  • Fluorescence recovery after photobleaching (FRAP) to measure protein diffusion, transport rates and association and dissociation kinetics
  • Fluorescence resonance energy transfer (FRET) to measure protein-protein interactions

Image Analysis Capabilities

Image Analysis Software

  • Software downloads
  • Wide variety of software for analysis of 2D images
  • Deconvolution of 3D images
  • Segmentation of nuclei and other structures from 3D images of tissue
  • Enumeration of fluorescence in situ hybridization (FISH) signals in the individual nuclei of tissue
  • Quantification of protein-protein co-localization in cell compartments. Live cell tracking
  • Visit collaborative research page for more information

Page last updated September 19, 2012 @ 1:47 pm