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Laboratory of Proteomics and Analytical Technologies (LPAT)

Providing qualitative, quantitative, and structural analysis via mass spectrometry, separations technology, and nuclear magnetic resonance

Laboratory Overview

The Laboratory of Proteomics and Analytical Technologies (LPAT) develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, to advance the understanding of cellular function at the proteomic and metabolomic levels. The LPAT specializes in the identification of individual proteins, characterization of entire protein complexes, and global analysis to discover changes in the proteomes of different samples. The laboratory also specializes in the development of custom assays for quantitation of specific molecules in complex clinical samples.  The technologies above are available as core services. Please see the LPAT Services page.

Mass Spectrometry Center (MS)

The LPAT Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for proteomic and metabolomic analysis. Areas of expertise include protein complex characterization, global and target protein quantitation, and identification of post-translational modifications. The Center also provides educational and training opportunities for scientists on the use, interpretation, and applications of MS technologies.

Nuclear Magnetic Resonance (NMR)

LPAT offers NCI researchers expertise in NMR experimentation and analysis using the most current hardware and software. The laboratory provides characterization of individual small molecules as well as metabolomic analysis for the comparative analysis of complex mixtures of metabolites in clinical samples such as serum and urine. Members of this group also have extensive experience in electronics engineering, pulse sequence programming, and database access.

Separations Technology (CE, HPLC, GC)

The LPAT has a repertoire of innovative methods for separation and quantitation of analytes using gas chromatography (GC), high-performance liquid chromatography (HPLC), and capillary electrophoresis (CE) online with various detection methods such as MS and UV. This group specializes in the development of highly accurate quantitative assays for specific molecules of interest. As with all areas within the LPAT, education and training in GC, HPLC, and CE is also offered to interested scientists.

Unparalleled Proteomic Services

Expertise: Besides being experts in MS, NMR and analytical science, many LPAT scientists have Ph.D.s in areas such as biochemistry and molecular biology.  They understand what type of results you require and strive to provide them quickly and efficiently.

Interaction: LPAT is located on the NCI-Frederick campus, allowing direct interaction with the scientists working on the project. LPAT scientists meet with you to carefully explain your results and they are available for any follow-up assistance that you may require, including assistance in manuscript writing.

Convenience: Samples can be sent to LPAT via the NCI courier.  We will even help you arrange shipping.  Budgeting issues are simple since no complicated invoices are necessary.

Page last updated January 17, 2013 @ 9:13 am