What’s New at LPAT
NEW! LPAT recently acquired a Tecan Freedom EVO robot for the analysis of clinical samples. MORE…
NEW! A study conducted by Steve Fox and Haleem Issaq in collaboration with DCEG investigators was highlighted in a recent edition of Chemical and Engineering News. This article highlighted their development of an assay to measure sarcosine, a potential diagnostic marker of advancing prostate cancer, in clinical samples. Unfortunately studies evaluating sarcosine as a biomarker have given conflicting studies prompting Steve and Haleem to speculate that some of these may have been measuring a combination of sarcosine and its structural isomers, α- and β-alanine. Indeed, when they replicated a previously reported study, they were able to show that a combination of three molecules was being mistaken for sarcosine. MORE…
The LPAT collaborates closely with NCI investigators as well as engages in technology development. The publications below highlight some of these activities.
Masood MA, Rao RP, Acharya JK, Blonder J, Veenstra TD. Quantitation of multiple sphingolipid classes using normal and reversed-phase LC-ESI-MS/MS: comparative profiling of two cell lines. Lipids 47(2):209-26, 2012.
Eliassen AH, Spiegelman D, Xu X, Keefer LK, Veenstra TD, Barbieri RL, Willett WC, Hankinson SE, Ziegler RG. Urinary estrogens and estrogen metabolites and subsequent risk of breast cancer among premenopausal women. Cancer Res. 72(3):696-706, 2012.
Wei BR, Simpson RM, Johann DJ, Dwyer JE, Prieto DA, Kumar M, Ye X, Luke B, Shive HR, Webster JD, Hoover SB, Veenstra TD, Blonder J. Proteomic Profiling of H-Ras-G12V Induced Hypertrophic Cardiomyopathy in Transgenic Mice Using Comparative LC-MS Analysis of Thin Fresh-Frozen Tissue Sections. J. Proteome Res. 11(3):1561-70, 2012.
Fuhrman BJ, Schairer C, Gail MH, Boyd-Morin J, Xu X, Sue LY, Buys SS, Isaacs C, Keefer LK, Veenstra TD, Berg CD, Hoover RN, Ziegler RG. Estrogen metabolism and risk of breast cancer in postmenopausal women. J. Natl. Cancer Inst. 104(4):326-39, 2012
Yan J, Mihaylov V, Xu X, Brzostowski JA, Li H, Liu L, Veenstra TD, Parent CA, Jin T. A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis. Dev. Cell 22(1):92-103, 2012
Auffray C, Caulfield T, Khoury MJ, Lupski JR, Schwab M, Veenstra TD. Looking back at genomic medicine in 2011.Genome Med. 4(1):9, 2012.
Puri A, Jang H, Yavlovich A, Masood MA, Veenstra TD, Luna C, Aranda-Espinoza H, Nussinov R, Blumenthal R. Material properties of matrix lipids determine the conformation and intermolecular reactivity of diacetylenic phosphatidylcholine in the lipid bilayer. Langmuir 2011 27(24):15120-8, 2011.
Kim HS, Vassilopoulos A, Wang RH, Lahusen T, Xiao Z, Xu X, Li C, Veenstra TD, Li B, Yu H, Ji J, Wang XW, Park SH, Cha YI, Gius D, Deng CX. SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity., Cancer Cell, 20(4):487-99, 2011.
Meyer TE, Fox SD, Issaq HJ, Xu X, Chu LW, Veenstra TD, Hsing AW. A reproducible and high-throughput HPLC/MS method to separate sarcosine from α- and β-alanine and to quantify sarcosine in human serum and urine. Anal Chem, 83(14):5735-40, 2011.
Johmura Y, Soung NK, Park JE, Yu LR, Zhou M, Bang JK, Kim BY, Veenstra TD, Erikson RL, Lee KS. Regulation of microtubule-based microtubule nucleation by mammalian polo-like kinase 1. Proc Natl Acad Sci U.S.A. 108(28):11446-51, 2011.
Johann DJ, Mukherjee S, Prieto DA, Veenstra TD, Blonder J.: Profiling solid tumor heterogeneity by LCM and biological MS of fresh-frozen tissue sections. Methods Mol Biol. 755:95-106,2011.
Zhang K, Fischer T, Porter RL, Dhakshnamoorthy J, Zofall M, Zhou M, Veenstra TD, Grewal SI: Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA. Science. 331(6024):1624-7, 2011.
Wei Q, Jiang H, Xiao Z, Baker A, Young MR, Veenstra TD, Colburn NH: Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. Proc. Natl. Acad. Sci. U.S.A. 108(17):7004-9, 2011.
Issaq HJ, Waybright TJ, Veenstra TD.: Cancer biomarker discovery: Opportunities and pitfalls in analytical methods. Electrophoresis. 32(9):967-75, 2011.
Bennuru S, Meng Z, Ribeiro JM, Semnani RT, Ghedin E, Chan K, Lucas DA, Veenstra TD, Nutman TB.: Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia. Proc. Natl. Acad. Sci. U.S.A. 108(23):9649-54, 2011.