Advanced Technology Program (ATP)

PCL Protocols and Resources

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  • Laboratory of Proteomics and Analytical Technologies (LPAT)
    • LPAT Services
      • LPAT Protein Complex Characterization
    • LPAT Protocols and Resources
      • Communication with the LPAT
      • LPAT FAQs
      • Timelines for Working with LPAT
        • Analysis of Gel Bands for Protein Identification
        • Comparative Analysis of Proteome Samples
        • NMR Analysis
        • Assay Development
      • Initiation of Projects with LPAT
      • Shipment of Samples to LPAT
        • NIH Main Campus Sample Shipment Instructions
        • Shipment Instructions for Off Campus Locations
    • LPAT Technology Development
      • Absolute Quantitation of Biologically Important Proteins in Clinical Samples
      • Clinical Proteomics
      • Development of MS Compatible Methods for Characterizing Protein-DNA Interactions
      • Dynamic Measurement of Proteins in Multiple Complexes
      • Isolation of Endogenous Protein Complexes via Epitope Tagging in ES Cells
      • Quantitative Proteomics of Cardiomyopathy in Thin Tissue Sections
      • Characterization of Highly Specific Protein Complexes
    • What’s New at LPAT
      • LPAT Installs Robot for Analysis of Clinical Samples
    • LPAT Lab Contacts
  • Protein Expression Laboratory (PEL)
    • PEL Services
      • Microfluidizer M-110EH and LV-1
      • High Yield Protein Synthesis Using Cell-Free Protein Expression System
    • PEL Protocols and Resources
      • Cedex Cell Analyzer
    • PEL Technology Development
      • Affinity Reagents for Analysis of the Human Cancer Proteome
      • Development of a multiplex onco-panel
      • Enhanced Protein Expression in Mammalian Cells
      • Expression System Evaluation and Optimization
      • High-throughput system for expression testing in E. coli using different mediums and temperatures
      • Improvements to Baculovirus Expression
      • Improving protein expression in the yeast, Kluyveromyces lactis
      • On Demand Protein Microarray
      • PEL Combinatorial Cloning Platform
      • Protein Expression Improvements
      • Tandem Affinity Purification (TAP)/Mass Spec Mediated Transient Protein Interaction
      • Technologies for Gene Engineering in Mammalian Cells
      • VLP mediated delivery of protein in mammalian cells
      • VLP mediated delivery of RNA to mammalian cells
    • What’s New at PEL
      • PEL Move
      • XMRV Protease Plasmid
    • PEL Lab Contacts
  • Protein Chemistry Laboratory (PCL)
    • PCL Services
      • Metabolic Analysis of Cells
      • Antibody Characterization
        • SPR Antibody Pairing
        • SPR antibody pairing data
      • Fluorescence Anisotropy
        • Protein – Nucleic Acid Interactions
        • Protein – Peptide Interactions
      • Schematic of an Imaging Mass Spectrometry experiment
    • PCL Protocols and Resources
      • Considerations before making fluorescence anisotropy measurements
      • Why is it important to understand the kinetics of my binding interaction?
      • Why N-terminal (Edman) sequencing ?
    • PCL Technology Development
      • Chemical deglycosylation of N,O-glycosylated proteins
      • Detergent free lysis of small amounts of cells and tissue samples
      • Immunoprecipitation/ Mass spectrometry assay (IPMS)
      • MALDI imaging in combination with LET technology
      • Mass Spectrometry based epitope mapping
      • Measurement of molecular interactions using Fluorescence Correlation Spectroscopy (FCS)
      • Metabolic analysis of cells using the Seahorse Biosciences Flux Analyzer XF-24
      • Surface capture of tagged proteins
      • Thermal protein denaturation to validate small molecule-protein interaction
      • Trehalose distribution in brain tissue by MALDI imaging
    • What’s New at PCL
      • PCL staff identify cleavage site of shed Mesothelin
      • PCL Staff Use NALDI-MS to Analyze Phospholipid Components of Liposomes
    • PCL Lab Contacts
  • Antibody Characterization Laboratory (ACL)
    • ACL Capabilities
    • ACL Protocols and Resources
    • ACL Technology Development
    • What’s New at the ACL
    • ACL Lab Contacts