Metabolic Analysis of Cells
The XF Extracellular Flux Analyzer from Seahorse Bioscience is fully-integrated instrument that simultaneously measures the two major energy yielding pathways – aerobic respiration and glycolysis – in a convenient, microplate format. The system is ideal for measuring metabolic changes in cells in response to ligand stimulation or drug treatment.
Using disposable fluorescent optical sensors and multiplexed fiber optics, the instrument offers:
- Simultaneously measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in as little as five minutes
- Perform time-resolved measurements of a single population of cells over a period of minutes, hours or even days
- Re-use cell samples after completing an XF measurement
The PCL now offers access to the XF Analyzer as a walk-up machine or as a complete service. Training, protocols and disposables (plates and sensors) are available through the PCL. Please contact Dr. Andrew Stephen (301-846-1634) for more information.
One of the standard assays that is performed in the training using the XF Analyzer is the Mitochondrial Stress Test. Inhibition of specific electron carrier complexes in the mitochondria can provide insightful information on basal respiration, ATP turnover, proton leak, and maximal respiration.
- Oligomycin inhibits Complex V
FCCP decouples the proton gradient
Rotenone/Antimycin A inhibits Complex III