PEL Technology Development
The purpose of technology development efforts in the PEL is to meet the cutting edge technology needs of the NCI and the scientific community as a whole through leveraging scientific expertise within the laboratory. A number of recent technology developments have lead to core services and scientific collaboration with NCI researchers that are reflected below or in our publications.
- Combinatorial Cloning Platform
- Technologies for gene engineering in mammalian cell lines
- RNA delivery using virus-like particles
- Affinity reagents for analysis of the human cancer proteome
- Development of a multiplex onco-panel
- Protein expression improvements
- Improvements to baculovirus expression
- On demand protein microarray
- Expression system evaluation and optimization
- Virus-like particle (VLP) mediated protein delivery in mammalian cells in vitro and in vivo
- Tandem affinity purification (TAP)/mass spec mediated transient protein interaction
