INTERNATIONAL STANDARD FOR BASIC FIBROBLAST GROWTH FACTOR

(RecDNA HUMAN TYPE)

90/712

AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of 0.5 ml of a solution that contained: 4 micro-g/ml bFGF; 10 mg/ml trehalose; 20 mmol/l sodium citrate, pH 5.0; 1 mmol/l EDTA.

UNITAGE
The assigned potency of the standard is 1600 International Units (IU) per ampoule.

CAUTION
All materials of biological origin should be regarded as potentially hazardous to health. THis material should be used and discarded according to your own laboratory safety procedures. This preparation is not for administration to humans.

USE OF AMPOULED MATERIAL
The standard is intended for calibration of local standards. For all practical purposes, each ampoule contains the same quantity of bFGF. The entire contents of each ampoule should be completely dissolved in a known volume of suitable solvent. It is recommended that, where possible, buffer containing carrier protein, rewarmed to approximately 37oC, should be used. No attempt should be made to weigh out any portion of the freeze-dried material.

STORAGE
Unopened ampoules should be stored at -20oC in the dark. For economy of use, it is recommended that the solution be subdivided into several small containers and stored at, or below, -40oC. Repeated freezing and thawing should be avoided. The ampoules do not contain bacteriostat and solutions of the ampouled material should not be assumed to be sterile.

CITATION
In all publications in which this preparation is used as an assay calibrant, it is important that the title of the preparation and ampoule code be cited correctly.

This reagent is available in small amounts (approximately 1 micro-g/sample) for use in the calibration of in vitro bioassays and in-house standards only and is not to be used for experimental purposes. Reference reagents are available to investigators with peer-reviewed support, at institutions or at commercial establishments and are limited to one sample of each reagent per year.