INTERIM REFERENCE PREPARATION OF NERVE GROWTH FACTOR (RecDNA HUMAN TYPE)

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AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of 1.0 ml of a solution that contained: 10 microgram/ml NGF; 2mg/ml trehalose; 5mg/ml human serum albumin; 10 mmol/l sodium citrate, pH 5.0.

UNITAGE
The assigned potency of the NGF reference preparation is 10,000 units per ampoule.

CAUTION
The preparation contains material of human origin (human serum albumin) which has been tested and found negative for HBsAG and antibody to HIV-1, HIV-1 and HCV. However, as with all preparations of human origin, this material cannot be assumed to be free from infectious agents. Moreover, the NGF, as for all materials of biological origin, should be regarded as potentially hazardous to health. The ampoule and its contents should be used and disposed of according to your own laboratory safety procedures. Such safety procedures will probably include wearing protective gloves and avoiding the generation of aerosols. Care should be exercised in opening the ampoules to avoid cuts. This preparation is not for administration to humans.

USE OF AMPOULED MATERIAL
For all practically purposes, each ampoule contains the same quantity of NGF. The entire contents of each ampoule should be completely dissolved in a known volume of suitable solvent. It is recommended that, where possible, buffer containing carrier protein should be used. No attempt should be made weigh out any portion of the freeze-dried material.

USE OF AMPOULED MATERIAL
Unopened ampoules should be stored at -20oC in the dark. For economy of use, it is recommended that the solution be subdivided into several small containers and stored at, or below, -40oC. Repeated freezing and thawing should be avoided. The ampoules do not contain bacteriostat and solutions of the ampouled material should not be assumed to be sterile.

CITATION
In all publications in which this preparation is used as an assay calibration, it is important that the title of the preparation and ampoule code be cited correctly.

This reagent is available in small amounts (approximately 10 microgram/sample) for use in the calibration of in vitro bioassays and in-house standards only and are not to be used for experimental purposes. Reference reagents are available to investigators with peer-reviewed support, at institutions or at commercial establishments and are limited to one sample of each reagent per year.