STEM CELL FACTOR/MAST CELL FACTOR (rDNA HUMAN)
- AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of 1 ml of a solution containing:
approximately 1 microgram stem cell factor/mast cell factor; 0.9% sodium chloride;
0.1% trehalose; 0.2% human serum albumin. The ampoules do not contain bacteriostat.
The assigned potency is 1,000 reference units (RU) per ampoule.
The preparation contains material of human origin which has been tested and found
negative for HBsAg and HIV antibody. However, as with all materials of
biological origin, this preparation should be regarded as potentially hazardous
to health. It should be used and discarded according to your own laboratory's safety
procedures. Such safety procedures probably will include the wearing of protective
gloves and avoiding the generation of aerosols. Care should be exercised in opening
ampoules or vials, to avoid cuts. This preparation is not for administration to
- USE OF AMPOULED MATERIAL
For all practical purposes, each ampoule contains the same quantity of the substances
listed above. Dissolve the total contents of the ampoule in 0.5 ml of sterile distilled
water. Rinse out the ampoule with a further 0.4 ml sterile distilled water and make
up the total amount to 1 ml. The concentration is then at 1,000 units per ml. For
further dilution, include carrier protein. No attempt should be made to weigh out
any portion of the freeze-dried material.
Unopened ampoules should be stored at -20oC. For economy of use, it is
recommended that the solution be sub-divided into several small aliquots and stored
at -40oC or below.
In all publications in which this preparation is used as an assay calibrant, it
is important that the title of the preparation and ampoule code be cited correctly.
This reagent is available in small amounts (approximately 1 microgram/sample) for
use in the calibration of in vitro bioassays and in-house standards
only and are not to be used for experimental purposes. Reference reagents are available
to investigators with peer-reviewed support, at institutions or at commercial establishments
and are limited to one sample of each reagent per year.