INTERNATIONAL STANDARD FOR INTERLEUKIN-1a (recDNA HUMAN TYPE)
AMPOULE CONTENTS: Each ampoule contains the residue
after freeze-drying of 0.5 ml of a solution that contained: 1.17 microgram IL-1a
3 mg trehalose; 0.5 mg human serum albumin; 50 mM sodium phosphate buffer
UNITAGE: The assigned potency is 117,000 international
units (IU) per ampoule.
CAUTION: The preparation contains material of human
origin (human serum albumin) which has been tested and found negative for HBsAg
and anti-HIV (LAV/HTLV III virus) antibody. However, as with all preparations of
human origin, this material cannot be assumed to be free from infectious agents.
Suitable precautions should be taken in the use and disposal of the ampoule and
its contents. This preparation is not for administration to humans.
USE OF AMPOULED MATERIAL: For calibration of national
standards/reference preparations, local, i.e. 'in house' standards and commercially
available materials. For all practical purposes, each ampoule contains the same
quantity of the substances listed above. Dissolve the total contents of the ampoule
in a known volume of suitable solvent (distilled water, saline or buffer) with carrier
protein where extensive dilution is required. No attempt should be made to weigh
out any portion of the freeze-dried material.
STORAGE: Unopened ampoules should be stored at -20oC.
For economy of use, it is recommended that the solution be sub-divided into several
small containers and stored at -40oC or below. The ampoules do not contain
bacteriostat and solutions of the ampouled preparation should not be assumed to
CITATION: The evaluation of this international standard
is described in the following paper: S. POOLE & R.E. GAINES DAS. The International
Standards for Interleukin-1Alpha and Interleukin-1Beta: Evaluation in an International
Collaborative Study. J. Immunol. Methods. 142, 1-13. (1991). In all publications
in which the 1st International Standard for Interleukin-1 alpha (code 86/632) is
used as an assay calibrant, it is important that the title of the preparation and
ampoule code be cited correctly.
SOURCE: The IL-1 contained in this ampoule was selected
from three preparations generously contributed by the Immunex Corporation, Dainippon
Pharmaceutical Co. and Biogen SA to the National Institute for Biological Standards
and Control (NIBSC), which is a WHO International Laboratory for Biological Standards.
This reagent is available in small amounts (approximately 1 microgram/sample) for
use in the calibration of in vitro bioassays and in-house standards
only and are not to be used for experimental purposes. Reference reagents are available
to investigators with peer-reviewed support, at institutions or at commercial establishments
and are limited to one sample of each reagent per year.