INTERIM REFERENCE PREPARATION OF INTERLEUKIN-5 (recDNA HUMAN TYPE)
- AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of.5 ml of a 0.15 M
solution of sodium chloride that contained: Approximately 0.5 microgram IL-5; 0.5
mg trehalose; 1.0 mg human serum albumin
The assigned potency is 5000 reference units (RU) per ampoule.
The preparation contains material of human origin (human serum albumin) which
has been tested and found negative for HB2Ag and anti-HIV (LAV/HTLV III
virus) antibody. However, as with all preparation of human origin, this material
cannot be assumed to be free from infectious agents. Suitable precautions should
be taken in the use and disposal of the ampoule and its contents. This preparation
is not for administration to humans.
- USE OF AMPOULED MATERIAL
For all practical purposes, each ampoule contains the same quantity of the
substances listed above. Dissolve the total contents of the ampoule in a known volume
of a suitable solvent (distilled water, saline or buffer) with carrier protein where
extensive dilution is required. No attempt should be made to weigh out any portion
of the freeze-dried material.
Unopened ampoules should be stored at -20oC. For economy of use,
it is recommended that the solution be sub-divided into several small containers
and stored at -40oC or below. The ampoules do not contain bacteriostat
and solutions of the ampouled preparation should not be assumed to be sterile.
In all publications in which this preparation is used as an assay calibrant,
it is important that the title of the preparation and ampoule code cited correctly.
This reagent is available in small amounts (approximately 1 microgram/sample) for
use in the calibration of in vitro bioassays and in-house standards
only and are not to be used for experimental purposes. Reference reagents are available
to investigators with peer-reviewed support, at institutions or at commercial establishments
and are limited to one sample of each reagent per year.