INTERIM REFERENCE PREPARATION OF INTERLEUKIN-8 (recDNA HUMAN TYPE)
- AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of 0.5 ml of a 0.15M
solution of sodium chloride that contained: approximately 1 microgram IL-8; 3 mg
The assigned potency is 1000 reference units (RU) per ampoule.
To avoid cuts, suitable precautions should be taken in the use and disposal of the
ampoule and its contents. This preparation is not for administration to humans.
- USE OF AMPOULED MATERIAL
For all practical purposes, each ampoule contains the same quantity of the substances
listed above. Dissolve the total contents of the ampoule in a known volume of a
suitable solvent (distilled water, saline or buffer) with carrier protein where
extensive dilution is required. No attempt should be made to weigh out any portion
of the freeze-dried material.
Unopened ampoules should be stored at -20oC. For economy of use, it is
recommended that the solution be sub-divided into several small containers and stored
at -40oC or below. The ampoules do not contain bacteriostat and solutions
of the ampouled preparation should not be assumed to be sterile.
In all publications in which this preparation is used as an assay calibrant, it
is important that the title of the preparation and ampoule code be cited correctly.
This reagent is available in small amounts (approximately 1 microgram/sample) for
use in the calibration of in vitro bioassays and in-house standards
only and are not to be used for experimental purposes. Reference reagents are available
to investigators with peer-reviewed support, at institutions or at commercial establishments
and are limited to one sample of each reagent per year.