Recombinant Human CD40 Ligand (CD40L)
 
Lot Number: 005355
NSC Number: D759060-X
Date of Manufacture: 9/9/97 (Immunex Corporation, Seattle, WA)
Amount Provided: 15 mg/mL, 1 mL per vial
Presentation: Frozen liquid
Description: Clear to slightly opalescent liquid when thawed. Product formulation is 15 mg/mL in 25mM Tris, 1% sucrose, 4% mannitol, 0.018% Tween-20, pH 7.4.
Product Testing:
(manufacturer CofA)
  1. pH (7.2)
  2. Particulate Matter (145 ≥ 10µm, 2 ≥ 25µm)
    A400 Assay (0.045 AU)
  3. RP-HPLC (4.3%, 47.9% and 47.8% components 1, 2 and 3)
  4. Osmolality (318 mOsm/kg)
  5. UV Spec Scan (14.87 mg/mL)
  6. CD40L Bioassay (7.3 x 104 U/mg)
  7. SDS-PAGE Scanning Densitometry (Pass for comparability to reference standard and 3 bands at 20 – 30 kDa (reduced))
  8. SEC-HPLC (10.4% high molecular weight component, 0% low molecular weight component)
  9. SDS-PAGE Silver Stain (Pass for comparability to reference standard)
  10. Western Blot (Pass for comparability to reference standard)
  11. Endotoxin / LAL Assay (< 1 EU/mg)
  12. Sterility, USP (No growth)
  13. General Safety Test (Pass)
  14. Rabbit Pyrogen Test (Pass; 0.6°C total temperature rise in 3 rabbits)
Product Storage: ≤ -70°C; Product is known to be stable at 2-80 C for 3 months
 
Address questions or comments to:
Biological Resources Branch
NCI-Frederick Cancer Research and Development Center
PO Box B
Building 1052, Room 253
Frederick, MD 21702
muszynskik@mail.nih.gov
 
REV 082911
 
For Research Use Only. CAUTION: NOT FOR USE IN HUMANS