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Recombinant Human CD40 Ligand (CD40L)
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Lot Number:
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005355
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NSC Number:
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D759060-X
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Date of Manufacture:
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9/9/97 (Immunex Corporation, Seattle, WA)
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Amount Provided:
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15 mg/mL, 1 mL per vial
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Presentation:
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Frozen liquid
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Description:
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Clear to slightly opalescent liquid when thawed. Product formulation is 15 mg/mL
in 25mM Tris, 1% sucrose, 4% mannitol, 0.018% Tween-20, pH 7.4.
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Product Testing:
(manufacturer CofA)
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- pH (7.2)
- Particulate Matter (145 ≥ 10µm, 2 ≥ 25µm)
A400 Assay (0.045 AU)
- RP-HPLC (4.3%, 47.9% and 47.8% components 1, 2 and 3)
- Osmolality (318 mOsm/kg)
- UV Spec Scan (14.87 mg/mL)
- CD40L Bioassay (7.3 x 104 U/mg)
- SDS-PAGE Scanning Densitometry (Pass for comparability to reference standard and
3 bands at 20 – 30 kDa (reduced))
- SEC-HPLC (10.4% high molecular weight component, 0% low molecular weight component)
- SDS-PAGE Silver Stain (Pass for comparability to reference standard)
- Western Blot (Pass for comparability to reference standard)
- Endotoxin / LAL Assay (< 1 EU/mg)
- Sterility, USP (No growth)
- General Safety Test (Pass)
- Rabbit Pyrogen Test (Pass; 0.6°C total temperature rise in 3 rabbits)
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Product Storage:
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≤ -70°C; Product is known to be stable at 2-80 C for 3 months
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