Antibody Specificity:  Human IgM

Isotype:   Murine IgG1

Process:  Clarified with filtration through gauze and 5.0 micron filters.  Precipitation with 20% sodium sulfate, dialysis against PBS, pH 7.4.  Heat inactivation at 56^o C, high speed centrifugation at 30,000 rpm for 3 hours, diluted to 19.2 mg/ml protein with sterile PBS and dispensed as 1.0 ml sterile filtered aliquots.

Concentration: 2.2 mg/ml total protein, 2.3 ml/vial

Buffer:  PBS, pH 7.4

Production Date:  8/99

Product Testing:

MAP:

Cell Line Negative

Purity:

96.3% by HPLC-SEC

Mycoplasma:

Cell Line Negative

Sterility:

Pass

Endotoxin:

<0.05EU/ml

Product Storage:  -20^oC

Cell Line Origin:  Dr. Ronald Levy, Stanford University School of Medicine(415-725-6425)

CAUTION: FOR RESEARCH USE ONLY.   NOT FOR DRUG OR DIAGNOSTIC USE.  NOT FOR USE IN HUMANS

1D12 is aliquoted in 5 mg amounts and is available to investigators with peer-reviewed support, at institutions, and at commercial establishments.  Use of these materials is limited solely to in vivo and in vitro basic research studies.

NOTE: Recipients of bulk cytokines and monoclonal antibodies will be required to sign a Material Transfer Agreement.  All International Orders are required to pay a packaging and handling fee. All recipients are required to providea a FedEx account number. All recipients are required to pay for shipping.