Antibody Specificity:   Human and murine interleukin 1 b (IL-1 b)

Isotype:   Murine IgG₁

Process:   Clarified with filtration through gauze and 5.0 micron filters. Precipitation with 20% sodium sulfate, dialysis against PBS, pH 7.4. Heat inactivation at 56^oC, high speed centrifugation at 30,000 rpm for 3 hours, diluted to 19.2 mg/ml total protein with sterile PBS and dispensed as 1.0 ml sterile filtered aliquots.

Concentration:   19.2 mg/ml total protein, 1.0 ml/vial

Buffer:   PBS, pH 7.4

Production Date:   8/90

Product Testing:

MAP:

All negative

Purity:

57% (murine IgG1)

Mycoplasma:

Negative

Sterility:

Negative

Endotoxin:

0.1 EU/ml

Product Storage:   -20^oC

Cell Line Origin:   Drs. Kouji Matsushima and Joost Oppenheim, National Cancer Institute - Frederick Cancer Research & Development Center (301) 846-1551

Recommended Starting Concentration:   1 microgram of 3ZD will detect 1-10 ng of human or murine by dot blot or Western blot analysis.

Reference:   Journal of Immunology, 140: 1895-1902 (1988).

CAUTION: FOR RESEARCH USE ONLY. NOT FOR DRUG OR DIAGNOSTIC USE. NOT FOR USE IN HUMANS.

NOTE: THE 3ZD MONOCLONAL ANTIBODY HAS AFFINITY TO HUMAN AND MURINE IL-1 b AND NOT IL-1 a. THE ANTIBODY WILL NOT NEUTRALIZE IL-1 a OR CROSS REACT WITH IL-1 a.

3ZD is aliquoted in 20 mg amounts and available to investigators with peer-reviewed support, at institutions, and at commercial establishments. Use of these materials is limited solely to 'in vivo' and 'in vitro' basic research studies.

NOTE: Recipients of bulk cytokines and monoclonal antibodies will be required to sign a Material Transfer Agreement. All International Orders are required to pay a packaging and handling fee. All recipients are required to providea a FedEx account number. All recipients are required to pay for shipping.