Biological Resources Branch
11B.11 MONOCLONAL ANTIBODY

Antibody Specificity:   Mouse IL-4

Isotype:   Rat IgG1

Buffer:   50mM Sodium Phosphate and 300mM Sodium Chloride, pH 6.

Lot Number:   L0412006

Inventory Number:    05060201

Concentration:    21.2 mg/ml, 0.27 ml/vial (total 5.7 mg/vial)

Product Storage:    -70oC

Product Testing:
MAP: Cell Line Negative Purity by HPLC: > 95%
Mycoplasma: Cell Line Negative Bioburden: 0 CFU/ml
Endotoxin: 1.54 EU/mg

Bioactivity:
In vitro:      0.9 ng/ml 11B.11 neutralizes 86.8% 500 pg/ml IL-4
       9.0 ng/ml 11B.11 neutralizes 96.3% 500 pg/ml IL-4
       90 ng/ml 11B.11 neutralizes >97% 500 pg/nl IL-4
 
In vivo:      5 mg of 11B.11 per week per mouse will significantly reduce endogenous IL-4 levels in most experimental situations.

Cell Line Origin:   Dr. William Paul, NIH

References:   Nature 315:33 (1985)

CAUTION: FOR RESEARCH USE ONLY.  NOT FOR DRUG OR DIAGNOSTIC USE.  NOT FOR USE IN HUMANS.

11B.11 is available to investigators with peer-reviewed support, at institutions, and at commercial establishments. Use of these materials is limited solely to in vivo and in vitro basic research studies.

NOTE: Recipients of bulk cytokines and monoclonal antibodies will be required to sign a Material Transfer Agreement that is auto-generated by our web program, following the electronic submission of an order. All International requesters are required to pay a packaging and handling fee. An invoice will be emailed for these charges. All recipients are required to provide a FedEx account number. All recipients are required to pay for shipping.