Event Series

RNA Biology Laboratory Seminar Series

Event Description

Seminar title: "tRNA-codon fit enables LARP4 activity for poly(A) tail length protection of ribosomal protein mRNAs." SUMMARY: The mRNA encoding La-related protein-4 (LARP4) contains a short region whose codons program it for translation-dependent instability. When replaced by synonymous codons that match tRNA levels, LARP4 mRNA and protein increase substantially. Overexpression of the lowest abundance cellular tRNA independently increases LARP4 which promotes the net lengthening of poly(A) tails of ribosomal protein mRNAs. LARP4 harbors a 'La module' homologous to the La protein which binds the UUU-OH 3'-termini of newly transcribed pre-tRNAs and protects them from untimely exonucleolytic digestion. LARP4 exhibits RNA 3' binding sensitivity to modifications of the terminal nucleotide 2' and 3'-OH groups similar to that previously demonstrated for La.

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RNA Biology Laboratory

Sponsored By

RNA Biology Laboratory