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Optimization of Ultrasound-Assisted Extraction, HPLC and UHPLC-ESI-Q-TOF-MS/MS Analysis of Main Macamides and Macaenes from Maca (Cultivars of Lepidium meyenii Walp)

  1. Author:
    Chen, Shu-Xiao
    Li, Ke-Ke [ORCID]
    Pubu, Duoji
    Jiang, Si-Ping
    Chen, Bin
    Chen, Li-Rong
    Yang, Zhen
    Ma, Chao
    Gong, Xiao-Jie

  2. Author Address

    College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China. 18340862946@163.com., College of Medical, Dalian University, Dalian 116622, China. like905219@163.com., Tibet Plateau Institute of Biology, Lhasa 850001, China. puduo@126.com., Tibet Plateau Institute of Biology, Lhasa 850001, China. tpibjiangsp@126.com., School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China. chenyishan@126.com., College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China. chenlirong@dlu.edu.cn., HPV Immunology Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. zhenyang2@yahoo.com., Tibet Plateau Institute of Biology, Lhasa 850001, China. wvoo@163.com., College of Medical, Dalian University, Dalian 116622, China. gxjclr@163.com.,
    1. Year: 2017
    2. Date: Dec 10
    3. Epub Date: 2017 12 10
  2. Journal: Molecules (Basel, Switzerland)
    1. 22
    2. 12
    3. Pages: 2196
  4. Type of Article: Article
  1. Abstract:

    Ultrasound-assisted extraction (UAE), using petroleum ether as the solvent, was systematically applied to extract main macamides and macaenes from Maca hypocotyls. Extraction yield was related with four variables, including ratio of solution to solid, extraction temperature, extraction time, and extraction power. On the basis of response surface methodology (RSM), the optimal conditions were determined to be the ratio of solution to solid as 10:1 (mL/g), the extraction temperature of 40 176;C, the extraction time of 30 min, and the extraction power of 200 W. Based on the optimal extraction method of UAE, the total contents of ten main macamides and two main macaenes of Maca cultivated in twenty different areas of Tibet were analyzed by HPLC and UHPLC-ESI-Q-TOF-MS/MS. This study indicated that UAE was able to effectively extract macamides alkaloids from Maca hypocotyls. Quantitative analysis showed that geographical origins, not ecotypes, played a more important role on the accumulation of active macamides in Maca.

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  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.3390/molecules22122196
  3. PMID: 29232875
  4. View record in Web of Science®
  5. WOS: 000419242400161
  6. PII : molecules22122196

Library Notes

  1. Fiscal Year: FY2017-2018
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