Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes

It has been proposed that in the early stages of human immunodeficiency (HIV) infection, before the loss of CD4(+) T cells, inhibition of IL-12 production from host antigen- presenting cells plays a critical role in the suppression of T- helper cell type 1 responses. Activation of the G(i)-protein- coupled high-affinity N-formyl peptide receptor by f-met-leu- phe and HIV-derived peptide T-20-suppressed IL-12 p70 production from human monocytes in response to both T-cell- dependent and T-cell-independent stimulation are reported. Activation of the low-affinity N-formyl peptide receptor by the HIV-derived F-peptide suppressed IL-12 production more modestly. This suppression was pertussis toxin sensitive and was selective for IL-12; the production of IL-10, transforming growth factor-p, and tumor necrosis factor-alpha was unaltered. The production of IL-12 p70 by dendritic cells was unaffected by these peptides despite functional expression of the high- affinity fMLP receptor. These findings provide a potential direct mechanism for HIV-mediated suppression of IL-12 production and suggest a broader role for G-protein-coupled receptors in the regulation of Innate immune responses. (Blood, 2001;97:3531-3536) (C) 2001 by The American Society of Hematology.

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