Skip NavigationSkip to Content
Return Return to Search Results

Human CD8(+) T cells store RANTES in a unique secretory compartment and release it rapidly after TcR stimulation

  1. Author:
    Catalfamo, M.
    Karpova, T.
    McNally, J.
    Costes, S. V.
    Lockett, S. J.
    Bos, E.
    Peters, P. J.
    Henkart, P. A.

  2. Author Address

    Henkart, PA, NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NCI, Lab Receptor Biol & Gene Express, NIH, Bethesda, MD 20892 USA. NCI, SAIC Frederick, Microscopy & Image Anal Lab, Frederick, MD 21702 USA. Netherlands Canc Inst, NL-1066 CX Amsterdam, Netherlands.
    1. Year: 2004
  2. Journal: Immunity
    1. 20
    2. 2
    3. Pages: 219-230
  4. Type of Article: Article
  1. Abstract:

    The chemokine RANTES is secreted rapidly after activation of human CD8(+) T cells, with a cycloheximide-resistant burst during the first hour. This pattern was observed in purified memory and effector phenotype CD8+ cells from blood as well as in blasts. In contrast, secretion of other chemokines and interferon-gamma by these cells was sensitive to cycloheximide and detectable only after a lag. Immunofluorescence microscopy of CD8(+) memory and effector cells and blasts showed RANTES present in intracellular vesicles that do not significantly colocalize with cytotoxic granule markers or other markers of defined cytoplasmic compartments. Immunoelectron microscopy confirmed that RANTES is stored in small vesicles distinct from the lysosomal secretory granules. RANTES(+) vesicles polarize rapidly in response to TcR engagement and are more rapidly depleted from the cytoplasm. These results show that CD8+ T cells have two distinct TcRregulated secretory compartments characterized by different mobilization kinetics, effector molecules, and biological function

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel