Skip NavigationSkip to Content
Return Return to Search Results

Calcium Signaling in Prostate Cancer Cells - Evidence For Multiple Receptors and Enhanced Sensitivity to Bombesin/Grp

  1. Author:
    Wasilenko, W. J.
    Cooper, J.
    Palad, A. J.
    Somers, K. D.
    Blackmore, P. F.
    Rhim, J. S.
    Wright, G. L.
    Schellhammer, P. F.

  2. Author Address

    Wasilenko WJ EASTERN VIRGINIA MED SCH DEPT MICROBIOL & IMMUNOL NORFOLK, VA 23510 USA EASTERN VIRGINIA MED SCH DEPT UROL NORFOLK, VA 23510 USA EASTERN VIRGINIA MED SCH DEPT PHARMACOL NORFOLK, VA 23510 USA EASTERN VIRGINIA MED SCH VIRGINIA PROSTATE CTR NORFOLK, VA 23510 USA NCI MOL ONCOL LAB FREDERICK, MD USA
    1. Year: 1997
  2. Journal: Prostate
    1. 30
    2. 3
    3. Pages: 167-173
  4. Type of Article: Article
  1. Abstract:

    BACKGROUND. Cellular calcium is an important second messenger for growth regulation. We sought to identify potentially important receptors on prostate tumor cells by screening over 20 agonists for their ability to increase intracellular free calcium ([Ca2+](i)) in several human prostate tumor cell lines. METHODS. Intracellular calcium mobilization was detected using fura-2. RESULTS. We found bombesin, GRP, ATP/UTP, lysophosphatidic acid, thrombin, endothelin, histamine, and bradykinin increased [Ca2+](i) in the advanced tumor cell lines DU-145, PC3, and PPC-1. Bombesin failed to elevate [Ca2+](i) in an immortalized human prostate cell line. Rank-order of potency studies suggested the presence of P-2U nucleotide receptors for ATP/UTP on prostate epithelial cells. Potency studies also revealed GRP>>bombesin>>neuromedin B at elevating [Ca2+](i) in responding tumor cells. CONCLUSIONS. These findings indicate that androgen independent prostate tumor cell lines express multiple receptors capable of elevating intracellular calcium, and suggest that GRP receptors may be selectively expressed and/or coupled to calcium signaling during prostate tumor progression. Calcium sensitive cellular events may therefore contribute to the progression of prostate cancer. (C) 1997 Wiley-Liss, Inc. [References: 50]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel