Skip NavigationSkip to Content
Return Return to Search Results

The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation

  1. Author:
    von Eyss, B.
    Maaskola, J.
    Memczak, S.
    Mollmann, K.
    Schuetz, A.
    Loddenkemper, C.
    Tanh, M. D.
    Otto, A.
    Muegge, K.
    Heinemann, U.
    Rajewsky, N.
    Ziebold, U.

  2. Author Address

    [Maaskola, Jonas; Rajewsky, Nikolaus; Ziebold, Ulrike] Max Delbruck Ctr Mol Med MDC, Berlin Inst Med Syst Biol, Lab Syst Biol Gene Regulatory Elements, D-13125 Berlin, Germany. [Schuetz, Anja; Heinemann, Udo] Max Delbruck Ctr Mol Med MDC, Prot Sample Prod Facil, D-13125 Berlin, Germany. [Loddenkemper, Christoph] Charite Campus Benjamin Franklin, Res Ctr ImmunoSci, Berlin, Germany. [Muegge, Kathrin] NCI, Lab Canc Prevent, SAIC Frederick, Frederick, MD 21701 USA.;Ziebold, U (reprint author), Max Delbruck Ctr Mol Med MDC, Berlin Inst Med Syst Biol, Lab Syst Biol Gene Regulatory Elements, Robert Roessle Str 10, D-13125 Berlin, Germany;uziebold@mdc-berlin.de
    1. Year: 2012
    2. Date: Feb
  2. Journal: Embo Journal
    1. 31
    2. 4
    3. Pages: 972-985
  4. Type of Article: Article
  5. ISSN: 0261-4189
  1. Abstract:

    for their dependence on the Retinoblastoma protein and their role in cellular proliferation. E2F3 is uniquely amplified in specific human tumours where its expression is inversely correlated with the survival of patients. Here, E2F3B interaction partners were identified by mass spectrometric analysis. We show that the SNF2-like helicase HELLS interacts with E2F3A in vivo and cooperates with its oncogenic functions. Depletion of HELLS severely perturbs the induction of E2F-target genes, hinders cell-cycle re-entry and growth. Using chromatin immmunoprecipitation coupled to sequencing, we identified genome-wide targets of HELLS and E2F3A/B. HELLS binds promoters of active genes, including the trithorax-related MLL1, and co-regulates E2F3-dependent genes. Strikingly, just as E2F3, HELLS is overexpressed in human tumours including prostate cancer, indicating that either factor may contribute to the malignant progression of tumours. Our work reveals that HELLS is important for E2F3 in tumour cell proliferation. The EMBO Journal (2012) 31, 972-985. doi: 10.1038/emboj.2011.451; Published online 13 December 2011

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1038/emboj.2011.451
  3. View record in Web of Science®
  4. WOS: 000300872100018

Library Notes

  1. Fiscal Year: FY2011-2012

Your Recently Viewed Publications:

Are Parallel Proliferation Pathways Redundant?
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel