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RAB19 Directs Cortical Remodeling and Membrane Growth for Primary Ciliogenesis

  1. Author:
    Jewett, Cayla E.
    Soh, Adam W. J.
    Lin, Carrie H.
    Lu,Quanlong
    Lencer, Ezra
    Westlake,Christopher
    Pearson, Chad G.
    Prekeris, Rytis

  2. Author Address

    Univ Colorado, Dept Cell & Dev Biol, Anschutz Med Campus, Aurora, CO 80045 USA.Natl Canc Inst Frederick, Lab Cell & Dev Signaling, Frederick, MD 21702 USA.
    1. Year: 2021
    2. Date: Feb 8
  2. Journal: Developmental Cell
  3. Cell Press
    1. 56
    2. 3
    3. Pages: 325-340.e8
  5. Type of Article: Article
  6. ISSN: 1534-5807
  1. Abstract:

    Primary cilia are sensory organelles that utilize the compartmentalization of membrane and cytoplasm to communicate signaling events, and yet, how the formation of a cilium is coordinated with reorganization of the cortical membrane and cytoskeleton is unclear. Using polarized epithelia, we find that cortical actin clearing and apical membrane partitioning occur where the centrosome resides at the cell surface prior to ciliation. RAB19, a previously uncharacterized RAB, associates with the RAB-GAP TBC1D4 and the HOPS-tethering complex to coordinate cortical clearing and ciliary membrane growth, which is essential for ciliogenesis. This RAB19-directed pathway is not exclusive to polarized epithelia, as RAB19 loss in non-polarized cell types blocks ciliogenesis with a docked ciliary vesicle. Remarkably, inhibiting actomyosin contractility can substitute for the function of the RAB19 complex and restore ciliogenesis in knockout cells. Together, this work provides a mechanistic understanding behind a cytoskeletal clearing and membrane partitioning step required for ciliogenesis.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.devcel.2020.12.003
  3. PMID: 33561422
  4. PMCID: PMC7880532
  5. View record in Web of Science®
  6. WOS: 000627119800009

Library Notes

  1. Fiscal Year: FY2020-2021
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