Neutrophil Monitoring Laboratory (NML)

Services

The Neutrophil Monitoring Laboratory provides dedicated support to NIAID, NIH.

• Dr. John I. Gallin, Director, Warren Grant Magnuson Clinical Center
• Dr. Harry L. Malech, Chief, Laboratory of Host Defenses
• Dr. Stephen M. Holland, Chief, Laboratory of Clinical Infectious Diseases

 

Specific assays of neutrophil function provided by the laboratory include:

1. Determination of O₂^- generation
   1. Quantitative determination using superoxide dismutase inhibitable reduction of cytochrome c
   2. Histochemical determination using nitroblue tetrazolium staining
   3. Flow cytometric determination using dihydrorhodamine 123
2. Bactericidal activity using Staphyloccoccus aureus
3. Adherence to plastic/coated surfaces
4. Neutrophil Granule enzyme release - myeloperoxidase, lactoferrin, gelatinase
5. Neutrophil surface marker analysis - whole blood and upregulation in vitro
6. Chemotaxis