What is recombineering?

Recombineering is in vivo genetic engineering, also known as homologous RECOMBInation-mediated genetic engiNEERING. “In vivo” is within a bacterial cell, usually E. coli or S. enterica, although recently recombineering has been done in other species, too. The genetic modifications are catalyzed by bacteriophage recombination proteins produced within the bacterium. Importantly, these phage functions are able to recombine DNAs containing short, 50 base, homologies. We typically use the bacteriophage λ Red recombination proteins for recombineering but other systems are available. 

To order Court Lab Reagents, please email Dr. Shyam Sharan with the Mouse Cancer Genetics Program (MCGP) at sharans@mail.nih.gov . More information about MCGP can be found here.

To order BRB Recombineering Reagents please click here.