References

  • Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. & Court, D. L. (2014). Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol 106, 1 16 1-1 16 39. CPMB 2014.pdf

    Sawitzke, J. A., Thomason, L. C., Bubunenko, M., Li, X., Costantino, N. & Court, D. L. (2013). Recombineering: Using Drug Cassettes to Knock out Genes in vivo. Methods Enzymol. 533, 79-102. MIE dsDNA 2013.pdf

    Datta, S., Costantino, N., Zhou, X. & Court, D. L. (2008). Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages. Proc. Natl. Acad. Sci. USA 105, 1626-1631. Datta-2008 PNAS .pdf

    Bubunenko, M., Baker, T. & Court, D. L. (2007). Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol 189, 2844-53. Bubunenko-2007 J. bact.pdf

    Datta, S., Costantino, N. & Court, D. L. (2006). A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109-115. Datta-2006 Gene.pdf

    Yu, D., Sawitzke, J. A., Ellis, H. & Court, D. L. (2003). Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate. Proc. Natl. Acad. Sci. USA 100, 7207-7212. PNAS2003-overlap.pdf

    Court, D. L., Swaminathan, S., Yu, D., Wilson, H., Baker, T., Bubunenko, M., Sawitzke, J. & Sharan, S. K. (2003). Mini-λ: a tractable system for chromosome and BAC engineering. Gene 315, 63-69. Court-2003 Gene.pdf

    Yu, D., Ellis, H. M., Lee, E. C., Jenkins, N. A., Copeland, N. G. & Court, D. L. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97, 5978-5983. Yu-2000 PNAS.pdf

    Li, X., Thomason, L. C., Sawitzke, J. A., Costantino, N., & Court, D. L. (2013). Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli. Nucleic Acids Res., 41, e204. Li-2013 NAR.pdf 

  • Chan, W., Costantino, N., Li, R., Lee, S.C., Su, Q., Melvin, D., Court, D.L., and Liu, P. 2007. A recombineering based approach for high-throughput conditional knockout targeting vector construction. Nucleic Acids Res 35(8): e64. high throughput.pdf

  • Li, X. T., Thomason, L. C., Sawitzke, J. A., Costantino, N. & Court, D. L. (2013). Bacterial DNA polymerases participate in oligonucleotide recombination. Mol. Microbiol. 88, 906-20. Polymerases and ssDNA recombination.pdf

    Sawitzke, J. A., Thomason, L. C., Bubunenko, M., Li, X., Costantino, N. & Court, D. L. (2013). Recombineering: Highly Efficient in vivo Genetic Engineering using Single-strand Oligos. Methods Enzymol. 533, 157-77. MIE 2013 ssDNA.pdf

    Sawitzke, J. A., Costantino, N., Li, X. T., Thomason, L. C., Bubunenko, M., Court, C. & Court, D. L. (2011). Probing Cellular Processes with Oligo-Mediated Recombination and Using the Knowledge Gained to Optimize Recombineering. J. Mol. Biol. Oligo optimization.pdf

    Li, X. T., Costantino, N., Lu, L. Y., Liu, D. P., Watt, R. M., Cheah, K. S., Court, D. L. & Huang, J. D. (2003). Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli. Nucleic Acids Res. 31, 6674-6687. Li-2003 Nuc acid res.pdf

    Costantino, N. & Court, D. L. (2003). Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants. Proc. Natl. Acad. Sci. USA 100, 15748-15753. Costantino-2003 PNAS.pdf

    Ellis, H. M., Yu, D., DiTizio, T. & Court, D. L. (2001). High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides. Proc. Natl. Acad. Sci. USA 98, 6742-6746. Ellis-2001 PNAS.pdf

  • Warming, S., Costantino, N., Court, D. L., Jenkins, N. A. & Copeland, N. G. (2005). Simple and highly efficient BAC recombineering using galK selection. Nucleic Acids Res. 33, e36. Warming-2005 Nuc acid res.pdf

    Swaminathan, S., Ellis, H. M., Waters, L. S., Yu, D., Lee, E.-C., Court, D. L. & Sharan, S. K. (2001). Rapid engineering of bacterial artificial chromosomes using oligonucleotides. Genesis 29, 14-21. Swaminathan-2001 Genesis.pdf

    Lee, E. C., Yu, D., Martinez de Velasco, J., Tessarollo, L., Swing, D. A., Court, D. L., Jenkins, N. A. & Copeland, N. G. (2001). A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73, 56-65. Lee-2001 Genomics.pdf

  • Thomason, L. C., Costantino, N., Shaw, D. V. & Court, D. L. (2007). Multicopy plasmid modification with phage λ Red recombineering. Plasmid 58, 148-158. Thomason-2007 Plasmid.pdf

  • Thomason, L. C., Oppenheim, A. B. & Court, D. L. (2009). Modifying bacteriophage lambda with recombineering. Methods Mol Biol 501, 239-51. Thomason-2009 Met MB.pdf

    Oppenheim, A. B., Rattray, A. J., Bubunenko, M., Thomason, L. C. & Court, D. L. (2004). In vivo recombineering of bacteriophage λ by PCR fragments and single-strand oligonucleotides. Virology 319, 185-189. Oppenheim-2004 Virol.pdf

  • Swingle, B., Markel, E., Costantino, N., Bubunenko, M. G., Cartinhour, S. & Court, D. L. (2010). Oligonucleotide recombination in Gram-negative bacteria. Mol. Microbiol. 75, 138-148. Swingle-2010 Mol micro.pdf

  • Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. & Court, D. L. (2014). Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol 106, 1 16 1-1 16 39. CPMB 2014.pdf

    Sharan, S. K., Thomason, L. C., Kuznetsov, S. G. & Court, D. L. (2009). Recombineering: a homologous recombination-based method of genetic engineering. Nat. Prot. 4, 206-223. Sharan-2009 Nature Protoc.pdf

    Sawitzke, J. A., Thomason, L. C., Costantino, N., Bubunenko, M., Datta, S. & Court, D. L. (2007). Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond. Methods Enzymol. 421, 171-199. MIE review.pdf

    Thomason, L., Court, D. L., Bubunenko, M., Costantino, N., Wilson, H., Datta, S. & Oppenheim, A. (2007). Recombineering: Genetic engineering in bacteria using homologous recombination. In Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K., eds.), Vol. 1, pp. Unit 16 p. 1-24. 6 vols. John Wiley & Sons, Inc., Hoboken, N.J. CurrentProtocols.pdf

    Thomason, L. C., Myers, R. S., Oppenheim, A., Costantino, N., Sawitzke, J. A., Datta, S., Bubunenko, M. & Court, D. L. (2005). Recombineering in Prokaryotes. In Phages: Their Role in Bacterial Pathogenesis and Biotechnology (Waldor, M. K., Friedman, D. I. & Adhya, S. L., eds.), pp. 383-399. ASM Press, Washinton, D.C. 

    Court, D. L., Sawitzke, J. A. & Thomason, L. C. (2002). Genetic engineering using homologous recombination. Annu. Rev. Genet. 36, 361-388. Courtetal2002.pdf

    Copeland, N. G., Jenkins, N. A. & Court, D. L. (2001). Recombineering: a powerful new tool for mouse functional genomics. Nat. Rev. Genet. 2, 769-779. Copeland-2001 Nat rev Genet.pdf

Using tetA-sacB.