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In vitro assembly of cubic RNA-based scaffolds designed in silico

  1. Author:
    Afonin, K. A.
    Bindewald, E.
    Yaghoubian, A. J.
    Voss, N.
    Jacovetty, E.
    Shapiro, B. A.
    Jaeger, L.

  2. Author Address

    [Afonin, Kirill A.; Yaghoubian, Alan J.; Jaeger, Luc] Univ Calif Santa Barbara, Dept Chem & Biochem, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA. [Bindewald, Eckart] NCI, Basic Sci Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Voss, Neil; Jacovetty, Erica] Scripps Res Inst, Automated Mol Imaging Grp, Dept Cell Biol, La Jolla, CA 92037 USA. [Shapiro, Bruce A.] NCI, Ctr Canc Res, Nanobiol Program, Frederick, MD 21702 USA.;Afonin, KA, Univ Calif Santa Barbara, Dept Chem & Biochem, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA.;shapirbr@mail.nih.gov jaeger@chem.ucsb.edu
    1. Year: 2010
    2. Date: Sep
  2. Journal: Nature Nanotechnology
    1. 5
    2. 9
    3. Pages: 676-682
  4. Type of Article: Article
  5. ISSN: 1748-3387
  1. Abstract:

    The organization of biological materials into versatile three-dimensional assemblies could be used to build multifunctional therapeutic scaffolds for use in nanomedicine. Here, we report a strategy to design three-dimensional nanoscale scaffolds that can be self-assembled from RNA with precise control over their shape, size and composition. These cubic nanoscaffolds are only similar to 13 nm in diameter and are composed of short oligonucleotides, making them amenable to chemical synthesis, point modifications and further functionalization. Nanocube assembly is verified by gel assays, dynamic light scattering and cryogenic electron microscopy. Formation of functional RNA nanocubes is also demonstrated by incorporation of a light-up fluorescent RNA aptamer that is optimally active only upon full RNA assembly. Moreover, we show that the RNA nanoscaffolds can self-assemble in isothermal conditions (37 degrees C) during in vitro transcription, which opens a route towards the construction of sensors, programmable packaging and cargo delivery systems for biomedical applications.

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External Sources

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  2. DOI: 10.1038/nnano.2010.160
  3. View record in Web of Science®
  4. WOS: 000281603400015

Library Notes

  1. Fiscal Year: FY2009-2010
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