Skip NavigationSkip to Content
Return Return to Search Results

Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell-Cell Contact

  1. Author:
    Jin, J.
    Sherer, N. M.
    Heidecker, G.
    Derse, D.
    Mothes, W.

  2. Author Address

    Jin, Jing, Sherer, Nathan M.; Mothes, Walther] Yale Univ, Sch Med, Sect Microbial Pathogenesis, New Haven, CT 06520 USA. [Heidecker, Gisela, Derse, David] NCI, HIV Drug Resistance Program, Frederick, MD 21701 USA.
    1. Year: 2009
  2. Journal: Plos Biology
    1. 7
    2. 7
    3. Pages: 1 e1000163-15 e1000163
  4. Type of Article: Article
  1. Abstract:

    We have investigated the underlying mechanism by which direct cell-cell contact enhances the efficiency of cell-to-cell transmission of retroviruses. Applying 4D imaging to a model retrovirus, the murine leukemia virus, we directly monitor and quantify sequential assembly, release, and transmission events for individual viral particles as they happen in living cells. We demonstrate that de novo assembly is highly polarized towards zones of cell-cell contact. Viruses assembled approximately 10-fold more frequently at zones of cell contact with no change in assembly kinetics. Gag proteins were drawn to adhesive zones formed by viral Env glycoprotein and its cognate receptor to promote virus assembly at cell-cell contact. This process was dependent on the cytoplasmic tail of viral Env. Env lacking the cytoplasmic tail while still allowing for contact formation, failed to direct virus assembly towards contact sites. Our data describe a novel role for the viral Env glycoprotein in establishing cell-cell adhesion and polarization of assembly prior to becoming a fusion protein to allow virus entry into cells.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1371/journal.pbio.1000163
  3. PMID: 19636361

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel