All tissue/cell lines, tumors, stem cells, and biological products originating off the NCI-Frederick
campus, including tissues/cell lines from the NIH campus, commercial suppliers such as the ATCC
(American Type Tissue Collection) and all animal serum products must be tested.
Samples will be submitted to the LASP Animal Health Diagnostic Laboratory (AHDL) as in the past,
but the AHDL will submit samples to RADIL each Wednesday. Samples received on Tuesday pm,
Wednesday, Thursday, or Friday will be sent the following week. For testing cultured cells, one
small cryovial of each sample with 1 x 107 cells per vial. Cells may be in the form of pellet, in spent
growth medium (preferred) or PBS. For testing non-cellular material, such as material extracted from cell
culture, submit one cryovial with 0.5 ml per sample.
An on-line submission form is available at the AHDL web
The form must be filled out completely. Questions or concerns regarding the on-line form can be
addressed to the AHDL at 301-846-1134.
Samples may be given to LASP Building Manager or taken directly to Building 429-AHDL, Monday
through Friday 8am until 4:00pm.
All investigators must pay for each line tested and must have an NCI-Frederick Cost Center Number. Investigators
may contact their Administrative Office for such information. Requests for pricing can be directed to:
Spontaneous tumors that arise within NCI-Frederick animals, provided that the cells are not collected during the
time of a virus outbreak, may be exempted from testing; however, when a disease outbreak is confirmed within an
NCI or NIH animal facility, all biological materials passaged in vivo within the previous six weeks should be
re-submitted for testing or discarded so that they are not re-introduced into animals. If the passaging history
or the parental origin is uncertain, the sample should be resubmitted and tested for the listed agents.
Yes, although rare, agents such Lymphocytic Choriomeningitis Virus (LCMV) and Reovirus may be transmitted from
humans to animals. These tissues need to be from documented HIV-negative and Hepatitis B negative patients.
MTBM-M/R Testing: 7 to 10 days
Fax results to the appropriate ACUC Coordinator.
Attach results to your ASP or if the ASP was already submitted, fax results to Bethesda ACUC Coordinator at 301-402-1276 and specify to which ASP they should be attached.
Attach results to your ASP or if the ASP was already submitted, fax results to Frederick ACUC Coordinator Angela M. Stahl at 301-846-6682 and specify to which ASP they should be attached.
General Information: There is no time limit on validity of results; however, investigators are encouraged to update testing periodically, such as every 10 years, as the sensitivity and agents screened are likely to increase over time.