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The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at previously unthinkable speeds and a fraction of their former cost. At the Sequencing Facility, NCI researchers are provided access to the latest technologies, with consultation and Q&A services available throughout the design and execution of sequencing projects.
PacBio Sequel II Sequencing
- Sequencing via single molecule, real-time (SMRT) technology allows rapid identification of long nucleotide chains.
- Two sequencing modes: continuous long reads (CLR, long insert > 20 kb) and accurate circular consensus sequences (CCS, short insert < 20 kb) for project flexibility.
- Polymerase read lengths averaging greater than 120,000 bases per molecule, with maximum read lengths > 200,000 bases for high fidelity circular consensus reads, facilitate genome assembly and mapping of repetitive regions.
- Amplification-free direct sequencing of individual molecules precludes PCR bias and artifacts.
- Minimal machine turnaround time provides flexibility in experimental and run design.
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