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The Pathology/Histotechnology Laboratory (PHL) provides comprehensive research support for animal health monitoring, biomarker discovery/validation, drug development, genomics, and proteomics for NIH and other government scientists on a cost recovered basis. PHL is currently organized as multiple process groups within the Laboratory Animal Sciences Program (LASP) of the Frederick National Laboratory for Cancer Research.
The Veterinary Pathology Section (VPS) offers integrated pathology services including consult/design of necropsy protocols, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray construction or laser micro dissection (LCM). The Histotechnology Laboratory (HL) offers complimentary support including: animal necropsy; hematology and clinical chemistry; tissue trim, processing and embedding; tissue micro array (TMA) construction and testing; microtomy and cryotomy; histological staining; molecular histology including enzyme stains, immunohistochemistry (IHC), RNAscope in situ hybridization (ISH); and “goal appropriate” target enrichment workflows from frozen/fixed animal and human tissues, paraffin and OCT tissue blocks, paraffin block cores, and precut glass or plastic membrane slides including yield and quality DNA, RNA, and protein assessments.
The PHL also supports a range of digital imaging options including gross photography and Leica-Aperio bright field and fluorescent digital whole-slide imaging (WSI). An associated Leica ESlide database facilitates digital slide conferencing via secure WebEx and integrated image analyses utilizing the Leica Aperio Genie Toolbox software, or Indica Labs Halo. The PHL also has a bank of control reagents available for collaborative research.
Blanket/annual budget request. Because the PHL is a high-volume core that processes 4,000+ work submissions from 300+ submitting groups annually it utilizes an option within the NAS request submission process that changes the level of review and approval from individual work requests to an annual budget for each which eliminates the need to submit a new request for each new sample submission. Once established, requestors are free to submit work via the established core-specific sample submission process including same-day provided that the funding source has not changed.
IMPORTANT NOTE: NCI Requesters seeking supplemental funding via the NCI-F Office of the Director Research Support (ODRS) Program, CCR, or CCR Office of Scientific Technology Research (OSTR) may not request this via a blanket budget request. Instead please submit a separate “ad hoc” NAS request as these follow a scope specific approval process.
To establish an annual blanket budget request for your group:
- Contact the PHL for help with an annual project estimate. Alternately visit the PHL Portal where records from past project submissions including annual cost summaries are available 24/7. https://ncifrederick.cancer.gov/Rtp/Lasp/Phl/Portal/.
- Select the blue BEGIN REQUEST Link located at the top right of this page to initiate a blanket/annual budget request to the PHL.
- Enter a NCI-F project identification (PID) account number. This optional field will specify the funding source of the blanket request. If this is the first time working with a FNLCR core service such that you lack a valid NCI-F PID, simply leave the field blank as it will become populated during the subsequent approval process. Otherwise, enter a valid NCI-F project ID to greatly speed processing. If you have more than one source of funding, you must submit a separate NAS request for each source.
- Enter a Project Name or Study ID. This free text field will help groups with more than one PID keep track of separate funding sources.
- Enter the Project Authorized Users (REQUIRED). This required field allows the PI to specify/list the staff that are authorized to submit work to the PHL on this account. Standard practice is to require every submitter have a unique account with documented authorization which facilitates reporting of work request by the various members of the lab group (e.g. different post doctoral fellows).
- Enter the FY PHL Annual Budget in US currency $$ (REQUIRED). Contact PHL staff for help with this. Alternately visit the PHL Portal where records from past project submissions including cost summaries are available 24/7. https://ncifrederick.cancer.gov/Rtp/Lasp/Phl/Portal/
- Enter the Funding Expiry date (MM/DD/YEAR, REQUIRED). Approved budgets automatically expire with the fiscal year (i.e. on the last Friday of September). It is however possible to set an earlier expiry to accommodate severable funding or the internal policies/financial practices of different NIH Institutes. Cancelation of an ongoing blanket PO is also possible provided work is not currently pending completion/billing.
- Enter a PHL Project Manager (OPTIONAL). Select from the list of contacts on the right side of this page.
- Complete the Request Submission. PHL staff will review the scope estimate and then return any revision to the budget for your approval. Once you confirm, the annual budget request will be routed through required fiscal and scope reviews resulting in a NAS approval number that must be included with each sample submission. If you have any questions, please call the PHL administrative office 301 846 1281.
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